Detection of Chrysanthemum Stunt Viroid by Dot Blot Hybridization Using DIG Labeled Probe
نویسندگان
چکیده
منابع مشابه
Sequence variability of Chrysanthemum stunt viroid in different chrysanthemum cultivars
Viroids are the smallest infectious agents, and their genomes consist of a short single strand of RNA that does not encode any protein. Chrysanthemum stunt viroid (CSVd), a member of the family Pospiviroidae, causes chrysanthemum stunt disease. Here, we report the genomic variations of CSVd to understand the sequence variability of CSVd in different chrysanthemum cultivars. We randomly sampled ...
متن کاملChrysanthemum stunt viroid: primary sequence and secondary structure.
The sequence of the 356 nucleotide residues of chrysanthemum stunt viroid (CSV) has been determined. Overlapping linear viroid fragments were obtained by partial ribonuclease digestion, radiolabelled in vitro at their 5'-ends, and sequenced using partial enzymic cleavage methods. Of the CSV sequence, 69% is contained in the published sequence of potato spindle tuber viroid (PSTV). Differences i...
متن کاملA Current Overview of Two Viroids That Infect Chrysanthemums: Chrysanthemum stunt viroid and Chrysanthemum chlorotic mottle viroid
The chrysanthemum (Dendranthema X grandiflorum) belongs to the family Asteraceae and it is one of the most popular flowers in the world. Viroids are the smallest known plant pathogens. They consist of a circular, single-stranded RNA, which does not encode a protein. Chrysanthemums are a common host for two different viroids, the Chrysanthemum stunt viroid (CSVd) and the Chrysanthemum chlorotic ...
متن کاملComplete Genome Sequences of Chrysanthemum Stunt Viroid from a Single Chrysanthemum Cultivar
The chrysanthemum stunt viroid (CSVd), a member of the genus Pospiviroid with a single circular RNA genome, infects many chrysanthemum species. Here, we report 25 complete genome sequences of CSVd in a single chrysanthemum cultivar, revealing 20 variants.
متن کاملInvasion of shoot apical meristems by Chrysanthemum stunt viroid differs among Argyranthemum cultivars
Chrysanthemum stunt viroid (CSVd) is a damaging pathogen attacking Argyranthemum plants. Our study attempted to reveal distribution patterns of CSVd in shoot apical meristems (SAM) and to explore reasons for differential ability of CSVd to invade SAM of selected Argyranthemum cultivars. Symptom development was also observed on greenhouse-grown Argyranthemum plants. Viroid localization using in ...
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ژورنال
عنوان ژورنال: Annual Report of The Kansai Plant Protection Society
سال: 1996
ISSN: 0387-1002
DOI: 10.4165/kapps1958.38.0_1